https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:50657 Wed 28 Feb 2024 16:28:37 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38759 in planta. BcNRAMP1 is expressed in the whole plant body of pak choi, with a higher abundance in root tissues than in shoots. Mn deficiency and Cd exposure strongly induced BcNRAMP1 transcription levels. Through transient expression of BcNRAMP1-GFP fusion protein in tobacco leaf epidermal cells, BcNRAMP1 was revealed as a plasma membrane protein. Expressing BcNRAMP1 in yeast enhanced yeast cells to absorb Mn, Cd, and iron (Fe). Overexpression of BcNRAMP1 in Arabidopsis wild-type and nramp1 mutant increased and complemented Mn and Cd transportation and accumulation, respectively. Using noninvasive microelectrode ion flux measurements, a direct evidence that BcNRAMP1 acts on Cd influx in Arabidopsis root cells was provided. The results of this study reveal that BcNRAMP1 functions as a NRAMP protein in planta, absorbing nutrient metal Mn and the toxic metal Cd.]]> Mon 24 Jan 2022 16:32:15 AEDT ]]>